Primer
Up RcnA

Part:BBa_K119007:Design

Designed by: Libertad Pantoja   Group: iGEM08_LCG-UNAM-Mexico   (2008-09-29)


Upper primer for RcnA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A prediction of the RcnR operator site was used to construct this part, we needed a repressor to control it, so we added the cI promoter upstream of the RcnR operator.


Source

Genomic DNA from E.coli and cI promoter.

References